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open source tools

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open source tools for minicubes

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Click here run our real time PCR simulation tool from FASTA sequences made as a Deepnote Application.

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Updated: 8:46 PM 11/9/2023

Description of update: Added HTML report output which contains primer design data of 10 sets of best primers with the PrimerDesign3 algoritm, sequence analysis, PCR simulation, melting point analysis, and capillary electrophoresis

The gallary below shows the input and output from the simulation tool

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We release our PCR process simulation tool which allows you to enter a sequence or a FASTA accession number from which we get the sequence via the NCBI Entrez API.

Hereafter we has an interface based on the algoritms of Primer3 for designing optimal primers and allowing setting min and max scores for the primers. The simulator will give you 10 set of primers with scores and

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The next step in the simulation is the PCR amplification based on the sequence and the primers. The simulation is based on our very rapid thermo-cycler engine in the RAPGNA device and it will illustrate for you how long time it takes to reach to every 5 cycles.

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Our CE simulator incorporate some inaccuracies that is given by the score of the primers, so low score primers will give broader CE peaks. The simulation compares a sample with a ladder.

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Get full control with the intuitive app

The powerful yet intuitive app gives you full control of your experiments from anywhere and accelerates every step of your PCR research. Shortening time between getting started and obtaining great results.

Access and control your Minicube PCR with the intuitive iPad app. From the powerful and user friendly interface, you can easily plan and setup protocols, control multiple devices at the same time – and even allow colleagues control of the same Minicube PCR from their apps.

The app works both online and off, and connects to your Minicube PCR via wifi or Bluetooth, allowing you full flexibility.

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Ready to get started?

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