RGX PATHOGEN WATCH
The RGX system is a fully automated biological detection system for unmanned surveillance of biological aerosols. The system performs automated aerosol capture, sample preparation, molecular analysis and detection. It includes an alarm module for CBRNE NATO communication.
FULLY AUTOMATED BIOLOGICAL DETECTION
Introducing the RGX System
A revolutionary device combining two decades of advancements in biological sample preparation and rapid qPCR technology into a single, state-of-the-art system. RGX leverages cutting-edge physics to deliver exceptional DNA/RNA extraction quality and ultra-fast multiplex qPCR diagnostics with multi-color capability for unparalleled accuracy and versatility.
The system features an innovative sensor technology with ultra-low noise performance, surpassing the limitations of traditional CMOS and CCD-based devices. This breakthrough enables incredibly rapid detection at remarkably low thresholds, redefining precision and efficiency in molecular diagnostics.
The system below is our field demonstrator with a limited cartridge for field testing and inbuild touch monitor for easy operations.
THE DETECTION PROCESS
The process is fully automatic and in reasonable concentrations of target material a test result can be obtained in less than 5 minutes.
THE CARTRIDGE
The cartridge contains all needed biochemicals for fully automated diagnostics and is a corner-stone development by GNACODE (patent pending) allowing fully automated sample preparation, qPCR and cleaning of the consumable back to a negative control. It contains chemicals for 10,000 sample prep and multiplex qPCR reactions.
RGX FIRMWARE STRUCTURE
The firmware structure consist of 8 major componets CORE, EAPI, OTA HMI, ADD, OIN, ALARM and UPDATE which are explained below. The front GUI system sends commands to the MCU of the RGX daughterboard in a simple and robust messaging format. The mainboard for data handling and communciation is based on a Raspberry Pi 5 Compute Module giving the device complete IOT properties as well as local options for fast networking between multiple units
RGX TOUCH
The touch interface gives a direct connection to the realtime firmware operating system on the RGX daughterboard. The GUI interface can be used to see statistics of operations, alarms, settings, current prototols and start and stop of operations.
RGNA CORE FIRMWARE
RGNA CORE represents 15 years of software development originating from the Minicube PCR and refinements of control of critical hardware components for rapid sample prep, milli-kelvin precision temperature control of heating and cooling cycles for qPCR, optical readout photon detection of multiple colors, and information gathering for a range of onboard sensors and controls of coupled actuators.
RGNA EAPI FIRMWARE
The RGNA Embedded Applicatio Programming Interface (EAPI) is the robust interface governing the intercommunication on the RAPGNA devices. This is the newest component in our firmware portfolio and equal to the CORE it originates from robust Linux communication from the Minicube platform. The messaging system runs on a RS485 bus between the CORE MCU and the dedicated MCU’s handling sampling, sample prep, liquid handling, waste management, optics and heating and cooling during PCR, and readout from onboard sensors.
RGNA OTA FIRMWARE
The RGNA Over The Air (OTA) running from our GNACODE.IO server originates from the IOT developments by Xtel (now Xtellio) for the Minicube PCR and represents cutting-edge secured communication with private/public keys and signed software update packages. The OTA firmware ensures integrity checks before applying updates and only authorizes our devices to do the updates.
RGNA HMI FIRMWARE
The RGNA Human Machine Interface (HMI) is a new development for the RAPGNA devices based on QT. Provides a streamlined user interface for running tests. Displays real-time device status, test progress, and results.Allows users to initiate tests and manage sample inputs. Admin Interface (Settings & Diagnostics). Provides access to advanced configuration settings.Enables firmware updates and system diagnostics.Allows detailed logging of errors and events and performs results handling & barcode printing where it generates results in a secure format. The encrypted barcodes can only be read by a secure app and ensures tamper-proof data integrity.
RGNA PHD FIRMWARE
RGNA Analysis and Data Display (ADD) Firmware is the analytical module providing rapid analysis and diagnostics of the lastest event, as well as statistics, trendlines etc across a number of detection events. The underlying influxDB database is extremely fast compared to standard SQL databases and gives millisecond access to data .
RGNA ALARM FIRMWARE
The RGNA ALARM Firmware is an IRQ driven alarm function coupled to sensor threshold levels, and as soon as a threshold on a sensor is passed an IRQ signal is send to various forms of visible, audible alarms or network signals for remote signalling. The ALARM messaging system operates independent IRQ wires on the electronics and provide nanosecond signalling speed.
RGNA UPDATE SERVER
The RGNA UPDATE Server is technically not part of the embedded firmware but is our company Github server or a customer defined server from where automatic updating of the system can be triggered.
RGNA OIN FIRMWARE
The RGNA Operations & Insights Nexus (OIN) is a cloud linked system for diagnostics, remote control and reporting which is under development. The short specification of this firmware module is:
- Encrypted Result Transmission
- Backend Surveillance System
RGX INFINITY FILTERS
The infinity filters of the RGX systems allows any excitation and emission wavelenght to be set between 320-850 nm giving full flexibility for multiplex design with any flourophores and for combining multiple assays with very different optical requirements. Here the system is set to oscillatate between 484 nm and 488 nm with a few nanometers bandwidth illustrating that the system can make very sharp and narrow filters.
MULTIPLEX EXCITATION
Here we show rapid excitation of 5 different bandwidths in just 551 ms. Showing the dynamics of the RGX infinity filter system. The system can create any bandwidth filter between 320-850 nm – making the optical system flexible for any flourophore design.