With a peak ramping speed of 9°C/sec we have completed a 35 cycles 2-stage PCR in 21 min and 40 sec. We have a modified firmware that can make 35 cycles of 2-stage PCR in just 16 minutes – we call this “relaxed mode” since we have reduced the precision from 0.020°C to 1.0°C
Minicube PCR is probably the best thermo-cycling device on the market
Sixteen pcr machines in one
With Minicube PCR, you’ll be able to run up to 16 different PCR protocols asynchronously at the same time.
Each of the Minicube PCR’s 16 wells is its own precisely controlled temperature zone. The individual control of each well provides complete flexibility that will change the way you work. Imagine running protocols on 8 tubes while incubating 8 other tubes at 8 different temperatures – adding them to the Minicube PCR on the fly.
Flexibility and optimization
The Minicube PCR machine is ideal for optimization of protocols, preparation for sequencing, cloning, multiple parallel incubations, PCR gradients and other use of protocols that normally would be run in series on standard equipment but now can be run in parallel.
The Minicube PCR is also ideal for sharing PCR optimizations, because it has no well-to-well variation and no machine-to-machine variation. This means dramatically reduced PCR run times for you, without compromising on precision and liability.
The speed of the machine allows you to run a 35 cycle PCR experiment in about 30 minutes. The heating ramp rate is >8 °C/sec and the cooling rate is >2.5°C/sec. Both the heating and cooling rate exceeds the physical limits of the temperature change in a 25 µl liquid sample.
Get full control with the intuitive app
The powerful yet intuitive app gives you full control of your experiments from anywhere and accelerates every step of your PCR research. Shortening time between getting started and obtaining great results.
Access and control your Minicube PCR with the intuitive iPad app. From the powerful and user friendly interface, you can easily plan and setup protocols, control multiple devices at the same time – and even allow colleagues control of the same Minicube PCR from their apps.
The app works both online and off, and connects to your Minicube PCR via wifi or Bluetooth, allowing you full flexibility.
Plan and organize your experiments on iPad – even from outside the lab
Control multiple machines in parallel
Share devices with your peers even at run time
Allow multiple users control of different tubes on the same machine
Easy setup via Bluetooth proximity sensing
Open source software available if you want more advanced functionality
The Minicube PCR has the ability to work as a router (without internet connection). If you have a wifi network in the lab you can easily connect both the Minicube machine and your Minicube App to the lab network.
For Bioinformatics users and molecular biologists skilled in programming it is worth noting that the communication between the Minicube App and the Minicube PCR is via a HTTP protocol called RESTful. There exist an Open-Source community writing apps on Linux and Android for the Minicube PCR. Not officially supported by GNAcode but our engineers do provide code examples.
High precision and accuracy
The Minicube PCR is constructed with the markets highest precision and accuracy and the well-to-well variation is confined to second decimal.
Each well is an individual heating and cooling element thermal isolated from the other wells in the device and monitored by A-class PT1000 sensor calibrated to NIST standards. All wells are synchronized to run the same ramps so all experiments are carried out with only a few milli-kelvins of difference.
Precision is defined as how well a temperature can be repeated and our precision is better than 0.005 °C (5 milli-kelvin). Accuracy is defined as how close to a NIST calibrate temperature our device is and our accuracy is better than 0.03 °C
Calibrated precision and accuracy
The machine was designed and calibrated to yield unmatched temperature precision following NIST standards. Each well is a closed, precisely controlled temperature zone monitored NIST calibrated A-class PT1000 sensor and controlled by a rapid microprocessor. Our calibration procedures yield standard deviations of less than 0.05 °C, and since all sensors are calibrated and the electronic circuit are loaded with the calibration curves we have reduced the well-to-well variation to second decimal (better than 0.02)
Each well is calibrated at different temperatures (from 15-99 °C) by submerging the device into a NIST calibrated Fluke heated bath with a temperature accuracy of ±0.001 °C. The precision measurement circuit is optimized for high accuracy 24 bit recording, noise filtering and environmental stability in a temperature range between 15 to 99 °C. High quality bias circuitry ensures long term stability.
Download our printer friendly Minicube PCR datasheet for further technical specifications.
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Top and bottom solid aluminium
Aluminium 5083 is known for exceptional performance in extreme environments. It is highly resistant to attack by both salt water and industrial chemical environments. The top and bottom parts of the machine are each cut from a single piece of EN/AC 5083 high quality aluminium.
The reminder of the device is made with AISI304 authentic non-magnetic stainless steel which is corrosion resistant and which typically is utilized for hospital surgical equipment.
Outdoor industrial powder coating
The powder coat is a lead-free TGIC-free coating is specifically designed to meet stringent requirements of the construction industry. It comes with a 10 year product warranty – subject to terms and conditions. It passes DIN EN ISO 11507 (UVA – 340) where alternating exposure of first 8 hours at 60 ºC UV and then 4 hours at 45 ºC condensation for 1000 hours testing gives no chalking, excellent gloss retention and colour stability.
Quality port interfaces at the back
At the back there is access to the quality electronic ports for LAN, USB stick, and USB-B connections. Additionally the WIFI radio emits its signal from the back providing 2.4 GHz 802.11 b/g/n radio and excellent RF performance. The WIFI module is a quality radio that ensures a stable connection
High endurance XLR power connector
The XLR connector for the 24V power input chosen for its high endurance and reliability. Once inserted it does not fall out. XLR connectors are a style of electrical connector, primarily found on professional audio, video, and stage lighting equipment where a disconnect can have high economical consequences and thus absolute reliability is a must.
White PEEK tube insert
The top surface insertion point of tubes is made of a white PEEK which is UV resistant and withstand all alcohols, acetone, oils e.g. mineral oil, toluene, formaldehyde, sodium Hydroxide and acids (except hydrofluoric acid which can dissolve glas and rocks, Carbolic Acid (Phenol),Chromic Acid, Nitric Acid above 10%) but also not compatible with fluorine and chlorine.
Embedded is a “mid-heater” that is an addition to the top-heater. The surface is normally kept at 100°C (adjustable) to prevent condensation at the mid of the tubes. The PEEK surface has a very low heat conduction coefficient that ensures tubes can be inserted and removed without significant heat transferred to the fingers.
Variable rubber contacts for tube tops
The top-heater is equipped with individual rubber spring cusions that accommodates both dome and flat caps without need for adjustment. The heater is normally kept at 120 °C (variable) which removes any trace of condensation in the top of the tubes. Evaporation can actually be checked by the calorimetri function of the Minicube PCR machine. As of today this requires the use of the Scipy interface but it will eventually be built into the App
Very quiet ventilation system
The Minicube PCR cools and heats at the same time and therefore uses a considerable amount of energy in doing so. That energy needs to be removed from the device. Despite that – it never exceeds 35 dB where we have noticed that our competitors calls less than 45 dB for “whispering”. If only a few wells are activated the noise is lower than 25 dB. The device sucks in air from the front and the back and exhaust it below the machine in all directions. It requires a few centimeter of free air at the front and the back to be able to fully ventilate the heat sink of the device. The fan is free from mechanical noises and is generally the quiest fan on the market.
See the data from August 2017 where the Minicube PCR was utilized at AAU University Hospital to amplify the LTR gene from HIV2. The device could created an detectable amount of DNA of only 10 copies within only 35 cycles. The bands produced are sharp and only a few basepair wide illustrating the excellent thermal control by the Minicube PCR
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Application note – Calorimetry
The Minicube PCR has a calorimetry function that currently only can be utilized via the Scipy (Scientific Python Open Source) interface. We have utilized this to monitor the metabolism of approximately 100 Tardigrades (bear animals) in a 100 ul tap water solution vs control.
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Application note – Calorimetry 2
The Minicube PCR has a calorimetry function that currently only can be utilized via the Scipy (Scientific Python Open Source) interface. We have utilized this to differentiate molecule composition in a 25 µl sample and to measure the volumen with sub-microliter sensitivity.
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GNAcode develops machines and interfaces that open new paths for your creativity, and sets your work routines free. Obtaining great results just got a lot easier.
GNAcode have been rethinking some of the basic life-science instruments. We have carefully analyzed the core functionalities and the daily work patterns. By combining this knowledge with the latest technologies we have achieved more functionality and better precision performed in less time compared to traditional equipment. Our series of machines and interfaces opens new ways for you to be more creative and free in your lab environment.
Minicube PCR is the first in a series of machines that enables researchers to access and share multiple machines via one interface. The small flexible machines are ideal for protocol optimizations and small scale investigations.
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A triple-gas cell incubator suitable for undisturbed cell culturing free of risks of contamination. Easy to disassemble and all critical parts are autoclavable. Do CO2, O2 or CO2/O2 experiments with N2 as third gas.
The real-time version of the small, innovative Minicube PCR. All the great features are retained and expanded with dual-color fluorescence detection. Also with the possibility to customize the filter-block.
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