The ultimate Diagnostics
Introducing the RAPGNA
A revolutionary device combining two decades of advancements in biological sample preparation and rapid qPCR technology into a single, state-of-the-art system. RAPGNA leverages cutting-edge physics to deliver exceptional DNA/RNA extraction quality and ultra-fast multiplex qPCR diagnostics with multi-color capability for unparalleled accuracy and versatility.
The system features an innovative sensor technology with ultra-low noise performance, surpassing the limitations of traditional CMOS and CCD-based devices. This breakthrough enables incredibly rapid detection at remarkably low thresholds, redefining precision and efficiency in molecular diagnostics.
THE DIAGNOSTIC PROCESS
The process is simple and straightforward and allows self-administration and self-diagnostics by the machine in an ATM style kind of operation. Since a test can be performed in less than 5 minutes it can be comfortable used in a doctors office without lab facilities.
Sample Collection from Oasis Diagnostics
The sample collectors from Oasis Diagnostics, Super-SAL™. Interfaced to the system through a syringe insert.
Cooled Cartridge
The system operates on biochemicals (mastermix, primers, and nanoparticles) stored in a cooled cartdrige. It contains 10,000 single integrated sample prep and multiplex qPCR reactions.
THE CARTRIDGE
The cartridge contains all needed biochemicals for fully automated diagnostics and is a corner-stone development by GNACODE (patent pending) allowing fully automated sample preparation, qPCR and cleaning of the consumable back to a negative control. It contains chemicals for 10,000 sample prep and multiplex qPCR reactions.
FIRMWARE STRUCTURE
The firmware structure consist of 6 major componets CORE, SCL, HMI, PHD and MOIN which are explained below.
RGNA CORE FIRMWARE
RGNA CORE represents 15 years of software development originating from the Minicube PCR and refinements of control of critical hardware components for rapid sample prep, milli-kelvin precision temperature control of heating and cooling cycles for qPCR, optical readout photon detection of multiple colors, and information gathering for a range of onboard sensors and controls of coupled actuators.
RGNA SCL FIRMWARE
The RGNA Smart Connectivity Layer (SCL) is the robust and adaptive networking protocol governing the intercommunication on the RAPGNA devices. This is the newest component in our firmware portfolio and equal to the CORE it originates from robust Linux communication from the Minicube platform. The messaging system operates a backbone of onboard network connected and dedicated MCU’s with end-to-end encryption and secure booting.
RGNA OTA FIRMWARE
The RGNA Over The Air (OTA) running from our GNACODE.IO server originates from the IOT developments by Xtel (now Xtellio) for the Minicube PCR and represents cutting-edge secured communication with private/public keys and signed software update packages. The OTA firmware ensures integrity checks before applying updates and only authorizes our devices to do the updates.
RGNA HMI FIRMWARE
The RGNA Human Machine Interface (HMI) is a new development for the RAPGNA devices based on QT. Provides a streamlined user interface for running tests. Displays real-time device status, test progress, and results.Allows users to initiate tests and manage sample inputs. Admin Interface (Settings & Diagnostics). Provides access to advanced configuration settings.Enables firmware updates and system diagnostics.Allows detailed logging of errors and events and performs results handling & barcode printing where it generates results in a secure format. The encrypted barcodes can only be read by a secure app and ensures tamper-proof data integrity.
RGNA PHD FIRMWARE
RGNA Precision Health Dashboard (PHD) Firmware is the Doctors admin screen interface that provides detailed information about the patient diagnostics. The system also has a second channel that provides a technician mode for detailed hardware-level analysis, and displays system health, sensor status, and error reports. Access to logs of device activity for troubleshooting and auditing.
RGNA SCL FIRMWARE
The RGNA Smart Connectivity Layer (SCL) is the robust and adaptive networking protocol governing the intercommunication on the RAPGNA devices. This is the newest component in our firmware portfolio and equal to the CORE it originates from robust Linux communication from the Minicube platform. The messaging system operates a backbone of onboard network connected and dedicated MCU’s with end-to-end encryption and secure booting.
RGNA OTA FIRMWARE
The RGNA Over The Air (OTA) running from our GNACODE.IO server originates from the IOT developments by Xtel (now Xtellio) for the Minicube PCR and represents cutting-edge secured communication with private/public keys and signed software update packages. The OTA firmware ensures integrity checks before applying updates and only authorizes our devices to do the updates.
RGNA MOIN FIRMWARE
The RGNA Medical Operations & Insights Nexus (MOIN) is a cloud linked system for diagnostics, remote control and reporting which is under development. The short specification of this firmware module is:
- Encrypted Result Transmission
- Backend Surveillance System
GNACODE.IO UPDATE SERVER ACTIVE STATUS
Dashboard Reporting
Collected data are reported by the RGNA MOIN firmware component connected to an online secure server which obeys regional laws as e.g. the Personal Information Protection and Electronic Documents Act (PIPEDA) in Canada. The Canada Covid Tracker is an example of such a server (not operated by GNACODE)
Live Canada Event Map
Event Log:
10 years of IOT development
It started with the Minicube PCR
The RAPGNA device and RGNA firmware developments arises from the large program undertaking in Denmark and manufacturing in Thailand where the Minicube PCR was developed as NIST calibrated asynchronous 16 channel PCR machine with no well-to-well variation and drift-free temperature control with less than 20 milli-kelvin variation.
Minicube PCR – 16 channel asynchroneous PCR
Our first IOT device developed in the years 2014 – 2018 was novel and innovative. The first IOT enabled PCR machine. We have carried the experience and knowledge forward into the RAPGNA device.
IOT Device
The Minicube PCR machine was the first PCR machine to use HTTP Restfull for communication allowing the device to be controlled by personal-held devices such as smartphones and tablets. The RAPGNA is based on the original Minicube Platform developed by an experienced IOT team which have worked for Apple and Samsung.
