open source tools
open source tools for minicubes
AEROSOL SAMPLING
Aerosol Interface to Prep and PqPCR
Direct Aerosol Analysis
We have 20 years of experience with aerosol capture and direct qPCR. We have made an open-source design that can be 3D printed and which provides an interface to an aerosol sample to the capillary on the Minicube Prep or Minicube PqPCR machines
The files are available here
Onshape offers free user registration and the possibility to make and edit public files.
Just register and you can make a copy of the aerosol connector files that you can edit as you want and 3D print
JYPYTER NOTEBOOK
Jupyter Notebook plot of Minicube PCR data
Jupyter Notebook
We support the Minicube devices with an open-source Python API that we have used to create some Jupyter Notebook tools for the machine. Jupyter Notebook executes in a browser
Github Download
Download the Jupyter Notebook scripts here
Requirements
Install Anaconda 64 bit from here
or alternatively you can use the Python API with Scipy which you can download here
Experience with Python is also needed and we have recently upgraded to Python 3 so make sure you not are running old Python 2.7 code or examples
PyQt6 - Crossplatform
Pycharm and Qtdesigner used to make GUI
PyQt6 installers for GUI apps
Using PyQT with QtDesigner and PyCharm we created fixed executables and ported libraries that can be packed by operative system dedicated installers. This can be done on Linux, MacOS and Windows offering cross platform functionalty
Github
Not yet on Github but coming soon, we need to pack the resources and upload them. We will do shortly
Requirements
PyQt6
Python 3
PyCharm
CxFreeze to make executables and to lock the python libraries into the applications lib folders so they dont get updated and suddenly your application wont work
Aerosol Capture and direct qPCR
Here we have data captured by the aerosol interface where the flow of air into the sample capillary suddenly experience a pressure drop and therefore the particles slow down significantly and they easily get captured by the surface interaction
The K value which is a resistance coefficient describing the pressure loss. We are going from a 0.2 mm interface to the 1.5 mm capillary and K is given by:
K=2[1−(D2/D1)^4]
The loss in pressure causes the particles of aerosols to deposit on the walls of the capillary, subsequent qPCR analysis shows a clear present of the bacteria in the aerosol
Get full control with the intuitive app
The powerful yet intuitive app gives you full control of your experiments from anywhere and accelerates every step of your PCR research. Shortening time between getting started and obtaining great results.
Access and control your Minicube PCR with the intuitive iPad app. From the powerful and user friendly interface, you can easily plan and setup protocols, control multiple devices at the same time – and even allow colleagues control of the same Minicube PCR from their apps.
The app works both online and off, and connects to your Minicube PCR via wifi or Bluetooth, allowing you full flexibility.
