Suitable kits for flow cytometry for downstream analysis of Nanolysis results
Fluorescence Kits for Studying Irreversible Electroporation
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LIVE/DEAD™ Viability/Cytotoxicity Kit
This kit provides a two-color fluorescence assay that allows for the simultaneous determination of live and dead cells, using calcein AM and ethidium homodimer-1 respectively. Ideal for assessing cell viability after irreversible electroporation.
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CellROX™ Oxidative Stress Reagents
These reagents are designed to measure reactive oxygen species (ROS) in live cells. The fluorescent probes can be used to evaluate oxidative stress, which is a common consequence of irreversible electroporation.
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Annexin V-FITC Apoptosis Detection Kit
This kit detects apoptosis by binding Annexin V-FITC to phosphatidylserine on the surface of apoptotic cells. It is useful for identifying cells undergoing apoptosis after irreversible electroporation.
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Propidium Iodide Staining Solution
Propidium iodide (PI) is a red-fluorescent nucleic acid stain that is impermeant to live cells but stains dead cells. It is commonly used to assess cell membrane integrity post-electroporation.
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SYTOX™ Green Nucleic Acid Stain
SYTOX™ Green is a high-affinity nucleic acid stain that easily penetrates cells with compromised membranes, making it excellent for detecting dead cells in a population of electroporated cells.
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